Exploring Protein-DNA Interactions: The Yeast One-Hybrid Assay Technique
Yeast one-hybrid (Y1H) screening is a widely used method for identifying proteins that bind to a target DNA sequence, such as a promoter or cis-regulatory element. It is commonly used to discover upstream transcription factors involved in gene regulation.
At Omics Empower, we provide yeast one-hybrid screening services to help researchers identify candidate DNA-binding proteins with reliable screening workflows, flexible sequencing options, and follow-up support for positive clones.
Identify DNA-binding proteins with optimized workflows and scalable screening support.
High-throughput screening swiftly identifies proteins interacting with a target.
Reliable results from yeast cell expression systems reflect in vivo conditions.
High sensitivity captures even weak or transient protein interactions.
Over 10 years of experience in yeast hybrid systems
Published collaborations in Science, Nature, and more
12-step quality control across the workflow
Ready-to-use library vectors and yeast strains included
End-to-end project guidance and fast technical support
From sample prep to bioinformatics insights
| Library parameters | Gateway method for building database | SAMRT method is used to build the database |
| Library size (E. Coli) | ≥1X10^7cfu | ≥5X10^6cfu |
| Average length of insert | ≥1000bp | ≥1000bp |
| Recombination rate | ≥95% | ≥90% |
Bait: Provide the bait promoter sequence or a constructed bait plasmid.
Library: Nuclear extract library plasmid.
Germany: Arnold-Graffi-Haus / D85 Robert-Rössle-Straße 10 13125 Berlin
United States: (CA) 2 Goddard, Irvine, CA 92618
United States: (IL) 8255 Lemont Rd, #1, Darien, IL 60561
Hong Kong: Room 618, Building 6, Hong Kong Science Park, Pak Shek Kok, Hong Kong
Germany: Arnold-Graffi-Haus / D85 Robert-Rössle-Straße 10 13125 Berlin
United States: (CA) 2 Goddard, Irvine, CA 92618
United States: (IL) 8255 Lemont Rd, #1, Darien, IL 60561
Hong Kong: Room 618, Building 6, Hong Kong Science Park, Pak Shek Kok, Hong Kong
Germany: Arnold-Graffi-Haus / D85 Robert-Rössle-Straße 10 13125 Berlin
United States: (CA) 2 Goddard, Irvine, CA 92618
United States: (IL) 8255 Lemont Rd, #1, Darien, IL 60561
Hong Kong: Room 618, Building 6, Hong Kong Science Park, Pak Shek Kok, Hong Kong
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