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How to Choose the Right Vectors for Membrane Yeast Two-Hybrid (MbY2H) Screening
Nov 24, 2025
How to Choose the Right Vectors for Membrane Yeast Two-Hybrid (MbY2H) Screening
Mapping protein–protein interactions (PPIs) among membrane proteins is a long-standing challenge in molecular biology. Traditional GAL4-based Yeast Two-Hybrid (Y2H) systems rely on nuclear localizati...
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10x Flex Q&A: What Single-Cell Researchers Should Know
Nov 17, 2025
10x Flex Q&A: What Single-Cell Researchers Should Know
The 10x Genomics Single Cell Gene Expression Flex (often called 10x Flex) was launched to make single-cell transcriptome profiling more accessible and sample-friendly.Unlike traditional 3′ or 5′ scR...
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Single-Cell Sequencing
A Complete Guide to Single-Nucleus RNA Sequencing (snRNA-seq)
Nov 06, 2025
A Complete Guide to Single-Nucleus RNA Sequencing (snRNA-seq)
Since the 19th-century formulation of cell theory, biologists have sought to decode how individual cells collectively shape the complexity of life. Every organ, tissue, and physiological function orig...
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Single-Cell Sequencing
Yeast Two-Hybrid (Y2H) Systems: From GAL4 to Split-Ubiquitin Assays
Oct 29, 2025
Yeast Two-Hybrid (Y2H) Systems: From GAL4 to Split-Ubiquitin Assays
OverviewThe yeast two-hybrid (Y2H) system is a genetic method used to detect protein–protein interactions (PPIs) in living yeast cells. The assay relies on reconstituting a transcription factor when ...
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yeast two-hybrid screening
When Protein and mRNA Levels Don't Match: What Next?
Oct 15, 2025
When Protein and mRNA Levels Don't Match: What Next?
In multi-omics research, it is not uncommon to encounter puzzling results: a protein shows high expression in proteomics, yet the transcriptomics data reveal little or no change at the mRNA level. Doe...
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yeast two-hybrid screening
Comparing Single-Cell Sequencing Platforms: How to Choose the Right Fit for Your Study
Oct 09, 2025
Comparing Single-Cell Sequencing Platforms: How to Choose the Right Fit for Your Study
Since 2009, single-cell sequencing has moved from a novel method to a standard approach in many areas of biology. Today, it is routinely applied in cancer research, neuroscience, immunology, and devel...
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Single-Cell Sequencing
A Complete Guide to 10x Xenium Probe Customization
Sep 30, 2025
A Complete Guide to 10x Xenium Probe Customization
10x Xenium is a fluorescence in situ hybridization–based spatial imaging platform. Currently, 10x offers 11 predesigned probe panels, divided into two categories: Xenium V1 and Xenium Prime 5K. Both ...
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Spatial Transcriptomics
Yeast One-Hybrid (Y1H) Assay: Principles, Systems, and Applications
Sep 24, 2025
Yeast One-Hybrid (Y1H) Assay: Principles, Systems, and Applications
Introduction to Yeast One-Hybrid (Y1H) TechnologyThe Yeast One-Hybrid (Y1H) assay was first developed by Li and colleagues in 1993, based on the principle of the Yeast Two-Hybrid (Y2H) system. Unlike ...
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Yeast One-Hybrid
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