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Yeast Two-Hybrid (Y2H) Systems: From GAL4 to Split-Ubiquitin Assays
Oct 29, 2025
Yeast Two-Hybrid (Y2H) Systems: From GAL4 to Split-Ubiquitin Assays
OverviewThe yeast two-hybrid (Y2H) system is a genetic method used to detect protein–protein interactions (PPIs) in living yeast cells. The assay relies on reconstituting a transcription factor when ...
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yeast two-hybrid screening
When Protein and mRNA Levels Don't Match: What Next?
Oct 15, 2025
When Protein and mRNA Levels Don't Match: What Next?
In multi-omics research, it is not uncommon to encounter puzzling results: a protein shows high expression in proteomics, yet the transcriptomics data reveal little or no change at the mRNA level. Doe...
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yeast two-hybrid screening
Comparing Single-Cell Sequencing Platforms: How to Choose the Right Fit for Your Study
Oct 09, 2025
Comparing Single-Cell Sequencing Platforms: How to Choose the Right Fit for Your Study
Since 2009, single-cell sequencing has moved from a novel method to a standard approach in many areas of biology. Today, it is routinely applied in cancer research, neuroscience, immunology, and devel...
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Single-Cell Sequencing
A Complete Guide to 10x Xenium Probe Customization
Sep 30, 2025
A Complete Guide to 10x Xenium Probe Customization
10x Xenium is a fluorescence in situ hybridization–based spatial imaging platform. Currently, 10x offers 11 predesigned probe panels, divided into two categories: Xenium V1 and Xenium Prime 5K. Both ...
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Spatial Transcriptomics
Yeast One-Hybrid (Y1H) Assay: Principles, Systems, and Applications
Sep 24, 2025
Yeast One-Hybrid (Y1H) Assay: Principles, Systems, and Applications
Introduction to Yeast One-Hybrid (Y1H) TechnologyThe Yeast One-Hybrid (Y1H) assay was first developed by Li and colleagues in 1993, based on the principle of the Yeast Two-Hybrid (Y2H) system. Unlike ...
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Yeast One-Hybrid
Yeast Two-Hybrid (Y2H) vs. AP-MS: Comparing Protein Interaction Detection
Sep 11, 2025
Yeast Two-Hybrid (Y2H) vs. AP-MS: Comparing Protein Interaction Detection
Understanding how proteins interact is essential for revealing the molecular mechanisms that drive life processes. Proteins rarely act alone; instead, they form transient contacts or stable complexes ...
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Yeast Two-Hybrid
Cross-Species Cell Type Annotation for Plant scRNA-seq with the OMG Browser
Sep 05, 2025
Cross-Species Cell Type Annotation for Plant scRNA-seq with the OMG Browser
If you struggle to label plant cell types because classic markers don’t translate across species or tissues, the OMG Browser offers a no-code workflow based on Orthologous Marker Groups (OMGs). It ma...
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scRNA-seq
What Is GEM-X 3′ v4 in Single-Cell RNA Sequencing and How Does It Improve Data Quality?
Aug 29, 2025
What Is GEM-X 3′ v4 in Single-Cell RNA Sequencing and How Does It Improve Data Quality?
Single-cell RNA sequencing (scRNA-seq) has transformed our ability to study complex tissues, tumor heterogeneity, and immune microenvironments. By measuring gene expression at cellular resolution, scR...
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scRNA-seq
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